The Use of Chitosan for Flocculation Recovery of Bacillus Biomass Grown on Dairy and Wine Industry Effluents

The downstream processing of efficient biomass-based microbial biopesticides is heavily reliant on obtaining the largest concentration viable cells in most cost-effective manner. goal this research was to assess ability chitosan flocculation recover bacterial Bacillus sp. BioSol021 biomass from broth after biological treatment wastewaters dairy and wine industries. Second-order factorial design models were used estimate effect mixing speed efficiency, settling velocity, antimicrobial activity against Aspergillus flavus, i.e., inhibition zone diameter. Response surface methodology followed by multi-objective optimization applying desirability function (DF) genetic algorithm (GA). optimum values for diameter cheese whey effluent 88%, 0.10 mm/s, 51.00 mm, respectively. In case winery flotation effluent, efficiency 95% velocity 0.05 while 48.00 mm. These results indicate that utilizing as a agent not only fits criteria effective processing, but also has synergistic antibacterial activity.